Studies on Human Haptoglobins. V

Immunogenetics of Human Haptoglobins

A B S T R A C T Using a radioimmunoassay method in conjunction with double diffusion studies, we characterized the antigenic determinants of the three Hp Bellevue phenotypes. An antigenic model based on these data indicates that each phenotype comprises a heterogeneous group of proteins with properties depending on their content of normal (hp,8) and of mutant (hp,8Bellevue) chains of haptoglobi...

The determination of haptoglobins in normal human serum.

Haptoglobins in chronic lymphatic leukaemia.

Haptoglobin phenotypes have been determined in 30 chronic lymphatic leukaemia patients and concentrations determined in 21 of these. The number ofHpl phenotypes is slightly raised but not significantly so compared with a control population and is the same as that in first-degree relatives. Haptoglobin concentrations show higher concentrations in Hpl than Hp2, as has previously been reported. It...

Serum Haptoglobins in Haemolytic States.

Serum haptoglobin levels have been studied in patients with a number of haemolytic disorders. In general, serum haptoglobins were low in patients with increased haemolysis. Values were reduced irrespective of whether haemolysis occurred intravascularly or extravascularly. The reduction was related to the amount of haemoglobin destroyed per day. Serum haptoglobins may be raised in certain neopla...

Vol. 72 ESTIMATION AND PURIFICATION OF HAPTOGLOBINS

1. A procedure for the rapid, accurate estimation of haptoglobin in serum and purified preparations is described, based on the peroxidase activity of haemoglobin in its complexes with haptoglobin. 2. Hydrogen peroxide, guaiacol and methaemoglobin reagents are used, under well-defined conditions which reduce the peroxidase activity of free haemoglobin essentially to zero. The reaction is followe...